1、 Basic principles
Spectral confocal microscopy is used for imaging and spectral analysis of samples. Confocal microscope is a high-resolution microscope that uses confocal imaging technology to image samples at different depths and obtain three-dimensional image information.
2、 Imaging process
Light source and focusing:
Use white light or multi wavelength mixed light as the light source.
Light forms dispersion through a special lens and shines onto the sample.
Focus a laser beam on the surface of the sample to excite the fluorescence signal emitted by the sample.
Spectral analysis:
Use a spectrometer to perform spectral analysis on the fluorescence signal emitted by the sample.
By performing spectral analysis on the fluorescence signals at different positions of the sample, spectral information of the sample at different positions can be obtained.
Confocal imaging:
Only light located on the optical axis can enter the spectrometer through the small hole of the spectral component.
Light that is not on the optical axis cannot pass through the small hole, ensuring the clarity of the imaging.
By adjusting the focus position, image information of different depths of the sample can be obtained.
3、 Measurement principle
The principle of spectral confocal measurement is to utilize the phenomenon of chromatic aberration, which refers to the different focal lengths of monochromatic light in a lens. By using special lenses to extend the focal halo range of different colored light, a special magnification color difference is formed. When the measured object is located at different distances, a precise wavelength of light will be focused onto the measured object. By measuring the wavelength of reflected light, the precise distance from the object being measured to the lens can be obtained.
4、 Technical features
High resolution: Spectral confocal technology can achieve high-resolution imaging and spectral analysis of samples.
High sensitivity: capable of detecting weak fluorescence signals, improving measurement accuracy.
3D imaging: It can obtain three-dimensional image information of the sample, providing more comprehensive sample information.
Strong adaptability: can be used for measuring samples of different materials and shapes, including transparent, semi transparent, and opaque materials.
5、 Application Fields
Spectral confocal technology has a wide range of applications in biomedical, materials science, environmental monitoring, and other fields. In the biomedical field, it can be used for imaging and spectral analysis of cells and tissues, providing strong support for disease diagnosis and drug development. In the field of materials science, it can be used for imaging and spectral analysis of material samples, helping researchers understand the microstructure and properties of materials. In the field of environmental monitoring, it can be used to monitor pollutants and harmful substances in the environment.
In summary, the working principle of spectral confocal microscopy is based on the imaging and spectral analysis principles of optical technology, using confocal microscopy and special lenses to achieve high-resolution imaging and precise measurement. This technology has many advantages and broad application prospects in multiple fields.